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Product Summary	The Human Collagen Type I Alpha 2 (COL1a2)ELISA Kit is a 4 hour solid phase ELISA designed to measure Target factor levels in sample.
Product name	Human Collagen Type I Alpha 2 (COL1a2)ELISA Kit
Catalogue No.	EIA07008h
Product Datasheet	Human Collagen Type I Alpha 2 (COL1a2)ELISA Kit Download Manual
Alias	COL1A2, OI4, collagen type I alpha 2, collagen type I alpha 2 chain, EDSCV, EDSARTH2，Collagen alpha-2(I) chain (Alpha-2 type I collagen) ELISA Kit,COL1A2ELISA Kit
Background	Collagen alpha-2(I) chain is a protein that in humans is encoded by the COL1A2 gene.This gene encodes one of the chains for type I collagen, the fibrillar collagen found in most connective tissues. Mutations in this gene are associated with osteogenesis imperfecta, Ehlers-Danlos syndrome, idiopathic osteoporosis, and atypical Marfan syndrome. Symptoms associated with mutations in this gene, however, tend to be less severe than mutations in the gene for alpha-1 type I collagen since alpha-2 is less abundant. Multiple messages for this gene result from multiple polyadenylation signals, a feature shared by most of the other collagen genes.
Human Collagen Type I Alpha 2 (COL1a2)ELISA Kit Data Examples
Human Collagen Type I Alpha 2 (COL1a2)ELISA Kit Recovery	Matrix	Recovery range(%)	Average(%)
	serum(n=5)	88-104	96
	EDTA plasma(n=5)	86-104	98
	heparin plasma(n=5)	85-103	96

Human Collagen Type I Alpha 2 (COL1a2)ELISA Kit Linearity	The linearity of the Human Collagen Type I Alpha 2 (COL1a2)ELISA Kit was assayed by testing samples spiked with appropriate concentration of and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
	Sample	1:2	1:4	1:8	1:16
	serum(n=5)	95-105%	85-101%	91-104%	95-102%
	EDTA plasma(n=5)	84-97%	91-99%	82-99%	85-96%
	heparin plasma(n=5)	85-99%	80-94%	81-91%	85-98%

Human Collagen Type I Alpha 2 (COL1a2)ELISA Kit Assay Precision
	Human Collagen Type I Alpha 2 (COL1a2)ELISA Kit Intra-Assay Precision			Human Collagen Type I Alpha 2 (COL1a2)ELISA Kit Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	40	40	40
Mean	29.1	123	531	32.8	128	495
CV%	6.5	4.1	3.5	8.5	5	6.7

Human Collagen Type I Alpha 2 (COL1a2)ELISA Kit name	96Tests	48Tests	Storage
Human Collagen Type I Alpha 2 (COL1a2)ELISA Kit antibody precoated plate	8×12	8×6	4/-20℃
Human Collagen Type I Alpha 2 (COL1a2)ELISA Kit Standards	2vial	1vial	4/-20℃
Human Collagen Type I Alpha 2 (COL1a2)ELISA Kit Biotinylated antibody(1:100)	1vial	1vial	4/-20℃
Human Collagen Type I Alpha 2 (COL1a2)ELISA Kit Enzyme conjugate(1:100)	1vial	1vial	4/-20℃
Human Collagen Type I Alpha 2 (COL1a2)ELISA Kit Enzyme diluent	1vial	1vial	4/-20℃
Human Collagen Type I Alpha 2 (COL1a2)ELISA Kit antibody diluent	1vial	1vial	4/-20℃
Human Collagen Type I Alpha 2 (COL1a2)ELISA Kit Standard diluent	1vial	1vial	4/-20℃
Human Collagen Type I Alpha 2 (COL1a2)ELISA Kit Sample diluent	1vial	1vial	4/-20℃
Human Collagen Type I Alpha 2 (COL1a2)ELISA Kit Washing buffer(1:25)	1vial	1vial	4/-20℃
Colour Reagent A	1vial	1vial	4/-20℃
Colour Reagent B	1vial	1vial	4/-20℃
Colour Reagent C	1vial	1vial	4/-20℃
Human Collagen Type I Alpha 2 (COL1a2)ELISA Kit Instruction	1set	1set	RT

【Human Collagen Type I Alpha 2 (COL1a2)ELISA Kit Necessary for testing their own test facilities and equipment】

1.Microplate reader(Main wavelength 450nm filter,sub wavelength 630nm filter).

2.Washer(adjustable amount of liquid injection to ensure that each well 350μl lotion without overflow).

3.Clean benches,biological safety cabinets,fume hoods.

4.High-precision single-channel dispenser(range 0.5-10μl-20μl,20-200μl,200-1000μl).

5.High-precision multi-channel plus liquid(8 or 12,the range of 50-300μl of).

6.37℃ incubator.

7.Low temperature centrifuge.

8.Refrigerators(4℃,-20℃,-86℃).

9.Analytical balance.

10.Scissors,tweezers,pliers,and so on.

11.Swirl mixing device,low-frequency oscillator,and so on.

【Human Collagen Type I Alpha 2 (COL1a2)ELISA Kit Necessary for testing their own testing supplies and reagents】

1.Centrifuge tube(capacity of 1.5ml,5ml,and so on).

2.Disposable tip(range of 0.5-10μl-20μl,20-200μl,200-1000μl).

3.Pure water or distilled water.

4.Coordinate paper.

5.Absorbent paper.

6.EDTA,sodium citrate,heparin

Human Collagen Type I Alpha 2 (COL1a2)ELISA Kit Summary of operating procedures

1.Prepare reagents,samples and standards.

2.Add the prepared sample and the standard reaction at 37℃ for 90 minutes.

3.Wash the plate ,add the biotinylated antibody working solution,37℃ for 60 minutes.

4.Wash three times to add the Enzyme working solution,37℃ for 30 minutes.

5.Wash five times,and then add the Colour Reagent solution,37℃ within 30minutes.

6.Add the Colour Reagent.

7.Microplate reader measured OD values within 30 minutes.

8.Calculate the factor content of specimens tested.

Human Collagen Type I Alpha 2 (COL1a2)ELISA Kit Sample preparation

Serum,The collected whole blood was allowed to stand at 4 degrees overnight or centrifuged(1000 x g)for 10 minutes. The supernatant was collected and stored at -20℃ or -80℃. Avoid repeated freeze-thaw cycles.

Plasma,Collect plasma using 5% sodium citrate(sodium Citrate:blood=1:9)as an anticoagulant. Centrifuge samples for 15 minutes at 1000×g at 2 - 8℃ within 30 minutes of collection. Plasma was immediately removed and tested,or stored at -20℃ or -80℃ after dispensing. Avoid repeated freeze-thaw cycles.

Urine,Aseptically collect the first urine of the day. Centrifuge to remove particulate matter,assay immediately or aliquot and store at ≤-20℃. Avoid repeated freeze-thaw cycles.

Tissue homogenates,The preparation of tissue homogenates will vary depending on the type of tissue. For this assay,tissue was rinsed in ice-cold PBS(0.01 mol/L,pH 7.2-7.6)to completely remove excess blood and weighed prior to homogenization. The tissue was cut into small pieces,a certain amount of PBS was added,homogenized with a high-speed homogenizer or other homogenizer. The resulting suspension was sonicated with an ultrasonic cell disruptor and the cell membrane was disrupted and centrifuged at 5000×g for 5 minutes. Immediately remove the supernatant and perform the assay,or dispense and store at ≤ -20℃.

For other types of samples,please contact us.

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