Development of a new sandwich ELISA kit.
Enlibio Biotech ELISA Kit developed by our company has achieved high-sensitivity detection and successfully solved the deficiencies of traditional competitive assay ELISA (low sensitivity and specificity). The traditional method is to use competitive method to detect small molecules. Due to the nature of small molecules, they cannot realize double antibody sandwiches, that is, there are not enough antigen binding sites to achieve the amplification effect of labeled antibodies, resulting in low sensitivity. The developed by our company is based on the combination of chemical groups of small molecules under relatively mild conditions, linking into long arm complex structures, and forming multiple antigen binding sites, thereby achieving sandwich ELISA and successfully achieving amplification effects. , thereby increasing the sensitivity.
The principle of the traditional competition method ELISA is to coat antibodies against small molecule antigens on the microtiter plate. When testing, add the sample to be tested so that the antigen to be tested in the sample binds to the antibody&bsp;the antibody on the microtiter plate. HRP enzyme-labeled antigen is then added. This antigen can also bind to the antibody on the microtiter plate. Since the amount of immobilized antibody on the microtiter plate is limited, the amount of antigen in the sample increases, the HRP enzyme-labeled antigen can bind. The smaller the coated antibodies, the two antigens compete for binding antibody. Subsequent addition of the substrate TMB of the HRP enzyme, TMB is catalyzed by the enzyme to undergo a color reaction. The more antigen present in the sample, the less HRP-labeled antigen remains in the well of the microtiter plate and the lighter the color development. The color rendering principle and standard curve are as follows:
Traditional Competition ELISA Traditional sandwich ELISA Novel sandwich ELISA