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【Mouse 25 Dihydroxy vitamin D(25 OH VD) ELISA Kit composition】
Mouse 25 Dihydroxy vitamin D(25 OH VD) ELISA Kit name	96Tests	48Tests	Storage
Mouse 25 Dihydroxy vitamin D(25 OH VD) ELISA Kit antibody precoated plate	8×12	8×6	4/-20℃
Mouse 25 Dihydroxy vitamin D(25 OH VD) ELISA Kit Standards	2vial	1vial	4/-20℃
Mouse 25 Dihydroxy vitamin D(25 OH VD) ELISA Kit Biotinylated antibody(1:100)	1vial	1vial	4/-20℃
Enzyme conjugate(1:100)	1vial	1vial	4/-20℃
Enzyme diluent	1vial	1vial	4/-20℃
Mouse 25 Dihydroxy vitamin D(25 OH VD) ELISA Kit antibody diluent	1vial	1vial	4/-20℃
Mouse 25 Dihydroxy vitamin D(25 OH VD) ELISA Kit Standard diluent	1vial	1vial	4/-20℃
Sample diluent	1vial	1vial	4/-20℃
Washing buffer(1:25)	1vial	1vial	4/-20℃
Colour Reagent A	1vial	1vial	4/-20℃
Colour Reagent B	1vial	1vial	4/-20℃
Colour Reagent C	1vial	1vial	4/-20℃
Instruction	1set	1set	RT

【Mouse 25 Dihydroxy vitamin D(25 OH VD) ELISA Kit Necessary for testing their own test facilities and equipment】
1.Microplate reader(Main wavelength 450nm filter,sub wavelength 630nm filter).
2.Washer(adjustable amount of liquid injection to ensure that each well 350μl lotion without overflow).
3.Clean benches,biological safety cabinets,fume hoods.
4.High-precision single-channel dispenser(range 0.5-10μl-20μl,20-200μl,200-1000μl).
5.High-precision multi-channel plus liquid(8 or 12,the range of 50-300μl of).
6.37℃ incubator.
7.Low temperature centrifuge.
8.Refrigerators(4℃,-20℃,-86℃).
9.Analytical balance.
10.Scissors,tweezers,pliers,and so on.
11.Swirl mixing device,low-frequency oscillator,and so on.

【Mouse 25 Dihydroxy vitamin D(25 OH VD) ELISA Kit Necessary for testing their own testing supplies and reagents】
1.Centrifuge tube(capacity of 1.5ml,5ml,and so on).
2.Disposable tip(range of 0.5-10μl-20μl,20-200μl,200-1000μl).
3.Pure water or distilled water.
4.Coordinate paper.
5.Absorbent paper.
6.EDTA,sodium citrate,heparin

【Mouse 25 Dihydroxy vitamin D(25 OH VD) ELISA Kit Summary of operating procedures】
1.Prepare reagents,samples and standards.
2.Add the prepared sample and the standard reaction at 37℃ for 90 minutes.
3.Wash the plate ,add the biotinylated antibody working solution,37℃ for 60 minutes.
4.Wash three times to add the Enzyme working solution,37℃ for 30 minutes.
5.Wash five times,and then add the Colour Reagent solution,37℃ within 30minutes.
6.Add the Colour Reagent.
7.Microplate reader measured OD values within 30 minutes.
8.Calculate the factor content of specimens tested.

For detailed steps,please read the paper instructions.
If you need COA and SDS,please email us!

Serum,The collected whole blood was allowed to stand at 4 degrees overnight or centrifuged(1000 x g)for 10 minutes. The supernatant was collected and stored at -20℃ or -80℃. Avoid repeated freeze-thaw cycles.

Plasma,Collect plasma using 5% sodium citrate(sodium Citrate:blood=1:9)as an anticoagulant. Centrifuge samples for 15 minutes at 1000×g at 2 - 8℃ within 30 minutes of collection. Plasma was immediately removed and tested,or stored at -20℃ or -80℃ after dispensing. Avoid repeated freeze-thaw cycles.

Urine,Aseptically collect the first urine of the day. Centrifuge to remove particulate matter,assay immediately or aliquot and store at ≤-20℃. Avoid repeated freeze-thaw cycles.

Tissue homogenates,The preparation of tissue homogenates will vary depending on the type of tissue. For this assay,tissue was rinsed in ice-cold PBS(0.01 mol/L,pH 7.2-7.6)to completely remove excess blood and weighed prior to homogenization. The tissue was cut into small pieces,a certain amount of PBS was added,homogenized with a high-speed homogenizer or other homogenizer. The resulting suspension was sonicated with an ultrasonic cell disruptor and the cell membrane was disrupted and centrifuged at 5000×g for 5 minutes. Immediately remove the supernatant and perform the assay,or dispense and store at ≤ -20℃.

For other types of samples,please contact us.

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